生物制药专业英语presentation幻灯片.pptVIP

In 1990 the recombinant（重组） expression vector of the exogenous gene （外源基因） was injected into the skeletal muscle（骨骼肌） of mice by Wolff, as a result, the recombinant expression vector of the gene was detected in one part of the muscle cells, and expression product of the gene induced immune response to produce the antibody. 1、Immune mechanisms of DNA vaccine 3、the Immunization of DNA vaccine 4、The advantages and disadvantages of DNA vaccine 5、The application status of DNA vaccine 2、The preparation of DNA vaccine Immune mechanisms of DNA vaccine 1. DNA vaccine invades the organism transcription and translation synthesis of exogenous antigen protein 1、let CD8+T cell differentiate to CTL(Cytotoxic T lymphocyte) and MTC(memory T cells) 2、cellular immune response 3、humoral immunity（体液免疫） 引自百度百科 The preparation of DNA vaccine 2. Selection of the objective gene 1、the ?specific?antigen DNA?coding sequence with immunological activity 2、correct reading frame 3、whether there is rare codons and?whether the??shear is correct in mammalian cells and other?factors antigenic characteristics antigen presentation and immunogenicity immunogenicity Select the appropriate carrier The carrier（载体） must be able to express objective gene efficiently in mammalian（哺乳动物） cells. Resistance genes usually with antibiotics select as selective marker. it also can integrate（融合） the gene which can encode （编码） specific protein on the carrier as immune adjuvant（佐剂） to enhance the immune response Expression vector of construction Connect the objective gene and carrier, construct a recombinant ,select the appropriate host amplified（扩增） recombinant, After the extraction yield recombinants using ion exchange chromatography（离子交换层析） for purification（纯化）, can get the recombinant DNA vaccine the Immunization of DNA vaccine 3. Injection of immune pathway Intramuscular（肌肉的） injection: The advantage of this method is simple and easy, but antigen presenting ability is weak because of muscle tiss