色谱作业解.pptVIP

4. A water sample is analyzed for traces of benzene using GC. Samples and standards are spiked with a fixed amount of toluene as internal standard. The following data are obtained: …… What is the concentration of benzene in the sample? 解：内标法——作图法定量 * 20. 在一根2m长的色谱柱上,分析一个混合物,得到以下数据:苯、甲苯、及乙苯的保留时间分别为1?20??, 2?2??及3?1??；半峰宽为6.33?? 8.73??, 12.3??，求色谱柱对每种组分的理论塔板数及塔板高度。 解：三种组分保留时间及理论塔板数分别为： t苯 = 1?20?? = 80?? n = 5.54?(80/6.33)2 = 887 H = L / n = 2/887 ? 0.23cm t甲苯 = 2?2?? = 122?? n = 1083.7 H ? 0.18 t乙苯 = 3?1?? = 181?? n = 1204.2 H ? 0.17 钨届背逝肚碘仑状取的稽聚雅粘惩彻乳腥踢浇堂垃迪呆