中药莲子心中总生物碱的提取及特征图谱研究要点.doc

本科毕业设计（论文） 学 院 轻工化工学院 专 业 制药工程 年级班级 2011级（3）班 学 号 3211001806 学生姓名 指导教师 2015年6 月 摘要 本实验是对中的化学成分进行分离，并对所得到的成分，确定。相信随着科学技术的进一步发展，也会有越来越多的相关研究，能够早日实现其最大的药用价值。 经过前期查阅文献从而确定本次实验方案，本实验通过此次实验所得到的实验数据，可以为成分的探索和技术开发提供基础，Abstract Objective： This experiment is toseparate the chemical composition of Plumula，and HPLC analysis the total alkaloids，which make sure the kind and composition of total alkaloids separate from separate. I believe that with the further development of science and technology , there will be more and more relevant research , as early as possible to achieve its maximum medicinal value. Method: After a preliminary literature review to determine this experimental program , This experiment crushed the Plumula（）.And combined the extract,concentrated it until we could’t smell the taste of alcohol, the concentrate was adjusted to pH=3 with 1% hydrochloric acid, then filtered with gauze. The filtrate was adjusted to pH=9 with ammonia, the solvent extraction with dichloromethane,concentrated the extracts, we got 200g dark yellow precipitate., which was the total alkaloids of plumula. After a HPLC analysis to the total alkaloids, explored the analytical conditions of HPLC, made sure the best of the analytical conditions of HPLC.The characteristic spectrum was bulit in the best of the analytical conditions of HPLC. Results: It was found that the kind of alkaloids in Plumula contained far more than reported in the literature through the result of characteristic spectrum. It indicated that the Plumula contains a large number of unknown structure of alkaloids. Conclusion:The experimental data obtained in this experiment may provided a theoretical basis for the exploration and technology odevelopment of Plumula total alkaloids, and lay the foundation for future study of the pharmacological effects . Keywords : Plumula, total alkaloids, the ch