PCR技术总论课件.pptVIP

Nearest Neighbor DH DNA (kcal/mol) DH RNA (kcal/mol) DS DNA (cal/mol); DNA DS RNA (cal/mol) AA or TT - 9.1 - 6.6 -24.0 -18.4 AT - 8.6 - 5.7 -23.9 -15.5 TA - 6.0 - 8.1 -16.9 -22.6 CA or TG - 5.8 -10.5 -12.9 -27.8 GT or AC - 6.5 -10.2 -17.3 -26.2 CT or AG - 7.8 - 7.6 -20.8 -19.2 GA or TC - 5.6 -13.3 -13.5 -35.5 CG -11.9 - 8.0 -27.8 -19.4 GC -11.1 -14.2 -26.7 -34.9 GG -11.0 -12.2 -26.6 -29.7 The values for DH and DS are shown in the table. As an example, a 1 μM solution of the probe mentioned above (AGGTCATTG) in a 150 mM solution has a predicted Tm of: ????????Tm = 1000(-7.8-11.0-6.5-5.6-5.8