金磊毕业论文改3教程分析.doc

本科毕业设计（论文） 题 目 院 系 生物与食品工程学院 年 级 　20 　专 业　食品 班 级 学 号 学生姓名 指导教师 权英 职 称 论文提交日期 201年5月20日 Extraction?and?the?Characteristic?Map?of?Volatile?Composition?of??Rape? Honey Abstract In this paper, headspace solid phase micro extraction rape honey extraction of volatile components by gas chromatography for 10 kinds of rape honey volatile compounds were analyzed to establish the characteristic chromatogram rape honey volatile components. Headspace solid phase micro-extraction side processing rape honey, screening extraction time, extraction temperature, NaCl amount, which is divided into four choices extraction temperature gradient, respectively 30 ℃, 40 ℃, 50 ℃and 60 ℃, extraction time Select four gradient, respectively 20min 25min 30min and 40min, the amount of sodium chloride and extracted into 0g, 0.5g, 1.0g and 1.5g, determine the optimal operating conditions by a gas chromatograph 10 brands the rape honey volatile components resolved to give chromatograms, and then digitize the chromatogram to identify internal reference peak, calculate retention, and follow certain standards to identify common peak establish rape honey characteristic spectrum data analysis . Finalize headspace solid phase micro extraction optimum conditions are: extraction temperature is 40 ℃, extraction time was 30 minutes, sodium chloride was added 1.0g, get honey under conditions characterized by a total of 10 peaks. Keywords:Rape Honey; Headspace solid phase micro extraction; Gas Chromatography; Characteristic map  目录 1绪论 1 1.1简述 1 1.2蜂蜜鉴定方法的研究进展 1 1.2.1 感官分析 1 1.2.2 荧光光谱 1 1.2.3 高效液相色谱 1 1.2.4 气相色谱技术 2 1.3课题研究内容 2 1.3.1 研究背景 2 1.3.2 研究内容 2 2 实验部分 3 2.1 实验样品 3 2.2 实验试剂 3 2.3 实验器材 3 2.4 实验方法 4 2.4.1 顶空固相微萃取提取油菜花蜜挥发性成分方法 4 2.4.2 气相色谱仪的操作流程 4 2.4.3 气相色谱条件 5 2.4.5 挥发性成分的最优提取条件和气相色谱图的获得 5 2.4.6 色谱图的数字化 5 2.5数据处理方法 6 3 结果和讨论 7 3.1 萃取条件的优化 7 3.2 共有内参考峰的确定 9 3.3 相对保留值的处理结果 13 3.4 不同油菜花蜂蜜样