vector概要.ppt

H Cloning vectors 载体的功能和应具备的特点 载体的功能 1.运送外源基因高效转入受体细胞 2.为外源基因提供复制能力或整合能力 3.为外源基因的扩增或表达提供条件 载体应具备的条件 1.具有对受体细胞的可转移性2.具有与特定受体细胞相适应的复制位点或整合位点3.长度尽可能小，以提高其载装能力4.具有多种单一的酶切位点5.具有合适的选择性标记 Cloning vectors H1 design of plasmid vectors H2 bacteriophage vectors H3 cosmids and YACs and BACs H4 eukaryotic vectors Design of plasmid vectors Characteristics of plasmid vectors Alteration of plasmid vectors Type of plasmid vectors Development of plasmid vector research Familiar plasmid vector Characteristics of plasmid vectors Independent from nuclear genome Autonomously replica