Translationbasics 翻译基础培训教程文件.pptVIP

Ribosomes Prokaryotic ribosome Eukaroytic ribosome Sedimentation of ribosomes The ?ribosome cycle“ Polyribosomes (polysome) Peptidyl transferase reaction tRNA binding sites Overview on translation initiation Ribosome binding site (Shine-Dalgarno sequence) on mRNA Internal start site are important for polycistronic mRNAs N-formyl methionine Initiation factors bound to the ribosome Sequence of events during initiation in prokaryotes Capping Translation initiation in eukaryotes Start codon identification in eukaryotes Circular polysomes in eukaryotes Circular polysomes Short upstream ORFs in eukaryotes Internal ribosome entry sites in eukaryotes (IRES) Overview of the elongation phase Binding of aa-tRNA to the A-site Codon recognition by the anticodon EF-Tu GTP hydrolysis in codon recognition Accommodation as the second recognition control step Translocation Release factors Action of release factors Ribosome recycling factor Spezielle Genetik und Molekularbiologie SS 2006 Mol. Biol. of the Cell Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Cell Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Kozak sequence at start codon: G/ANNAUGG Mol. Biol. of the Gene Mol. Biol. of the Cell Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene EF-Tu: protection of ester from hydrolysis check for correct codon recognition Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Mol. Biol. of the Gene Spezielle Genetik und Molekularbiologie SS 2006