张玲香第次专业英语优秀培训书.pptVIP

Molecular Cytogenetic Characterization of a New Wheat- Thinopyrum intermedium Partial Amphiploid Resistant to Powdery Mildew and Stripe Rust Name: Zhanglingxiang Major: Biological technology Grade:Grade10 Class:Class1 Study purpose Plant material Research contents Research methods Research results Discussion Further research Purpose Partial amphiploids are useful in studying genomic affinities among alien chromosomes. They can be used to develop chromosome addition,substitution and translocation lines with the aim of transferring beneficial traits from Thinopyrum ssp. Into wheat. In this study, we determined the genomic composition of a partial amphiploid TE253 by GISH probed with St genomic DNA from Ps. strigosa and evaluated its resistance to both powdery mildew and stripe rust as well. Plant materials Th. intermedium, Ps. strigosa the partial amphiploid TE253 the common wheat varieties :Yannong 15, Chinese Spring (CS), Mingxian169 and Huixianhong The method of the study 1.Cytogenetic Analyses Seeds were germinated at 25°C → maintained at 4°C for 24 h→ transferred to 25 °C→Roots 1–2 cm were cut and treated in ice water for 24 h → fixing in Carnoy’s solution →stain and squash the root tips in carbol fuchsin→ observe mitotic chromosomes The method of the study 1.Cytogenetic Analyses (2)sample the spikes and anthers → fixed in Carnoy’s solution → dissociate in 1 M HCl at 60 °C for 6–8 min → squash in 1% acetocarmine. 2.Reactions to Powdery Mildew and Stripe Rust Race 15, a dominant local isolate of B. graminis f.sp.tritici , was used to test the seedling stage reaction in the greenhouse and a mixture of isolates was used at the adult stage in the field. A mixture of P. striiformis races Tiaozhong 29, 30, and 31 and Shuiyuan 46 was used at the jointing stage to evaluate resistance to stripe rust with the cultivar Huixianhong as a susceptible control. 3.Genomic in situ Hybridizat