macrophagemigrationinhibitoryfactorintheparaventricular.ppt

Macrophage Migration Inhibitory Factor in the Paraventricular Nucleus Plays a Major Role in the Sympathoexcitatory Response to Salt by Eduardo Colombari, Debora S.A. Colombari, Hongwei Li, Peng Shi, Ying Dong, Nan Jiang, Mohan K. Raizada, Colin Sumners, David Murphy, and Julian F.R. Paton Hypertension Volume 56(5):956-963 November 1, 2010 Copyright ? American Heart Association, Inc. All rights reserved. Figure 1. Colocalization of AVP and MIF in the PVN. Top, Low-power representative fluorescence images of the PVN showing endogenous MIF (green) and AVP (red) immunoreactivities and their colocalization (orange) at 3 different levels (?1.60, ?1.88, and ?2.12 mm relative to the bregma). Eduardo Colombari et al. Hypertension. 2010;56:956-963 Copyright ? American Heart Association, Inc. All rights reserved. Figure 2. Hyperosmotic stimulation induces MIF mRNA expression in the PVN via an AT1R-dependent process. Eduardo Colombari et al. Hypertension. 2010;56:956-963 Copyright ? American Heart Association, Inc. All rights reserved. Figure 3. Increased MIF expression in the PVN decreases HS-evoked sympathoexcitation. Eduardo Colombari et al. Hypertension. 2010;56:956-963 Copyright ? American Heart Association, Inc. All rights reserved. Figure 4. HS-evoked sympathoexcitation is reduced by MIF and augmented by [C60S]-MIF expression in the PVN. Sympathoexcitation was measured as the percentage increase (peak response) from baseline over the 40-second period of HS (A), the total increase above baseline (B), and the duration of the response (C). Eduardo Colombari et al. Hypertension. 2010;56:956-963 Copyright ? American Heart Association, Inc. All rights reserved. Figure 5. Localization of MIF transduction in the PVN. A, The center of the injection sites for eGFP, MIF, and C60S-MIF within the PVN are indicated. Eduardo Colombari et al. Hypertension. 2010;56:956-963 Copyright ? American Heart Association, Inc. All rights reserved. Figure 6. AAV2-CBA-MIF–induced expression of