姜黄素体外抗氧化活性的课稿.doc

No. 2013013 药学系学生毕业论文 论文题目：姜黄素体外抗氧化活性的研究 姓 名： 学 号： 院 系： 药学系 专 业： 药学 导师姓名： 二O年 六 月 目 录 中文摘要…………………………………………………………1 英文摘要…………………………………………………………2 研究论文…………………………………………………………3 前言…………………………………………………………3 材料与方法…………………………………………………3 结果…………………………………………………………5 附表…………………………………………………………6 附图…………………………………………………………7 讨论…………………………………………………………8 结论…………………………………………………………10 参考文献……………………………………………………10 综 述…………………………………………………………11 致 谢…………………………………………………………17 个人简历…………………………………………………………18 姜黄素体外抗氧化活性的研究 刘 阔 河北北方学院药学系药学专业2009级 摘要：目的：研究姜黄素的体外抗氧化作用。方法：用紫外分光光度法测定姜黄素对H2O2诱导的小鼠红细胞溶血和Fe2+-Vit C诱导的肝线粒体肿胀程度的抑制作用及结合TBA法测定姜黄素对由Fe2+-Vit C诱导的脂质过氧化或肝脏自氧化所导致的丙二醛（MDA）H2O2诱导的小鼠红细胞氧化溶血,也能抑制Fe2+-Vit C诱导的肝线粒体肿胀，并能减少肝匀浆中MDA的含量。结论：姜黄素具有较强的体外抗氧化活性。 关键词：姜黄素；体外抗氧化 The antioxidant activities of curcumin in vitro Liu Kuo pharmacy Specialty,Grade 2009 Department of pharmacyHebei North University ABSTRACT: Objective: To evaluate the anti-oxidative activities of curcumin in vitro. Methods: Using UV spectrophotometry method to determine the inhibition of curcumin on H2O2 induced hemolysis of red blood cells in mice, and the inhibition of liver mitochondria swelling induced by Fe2+-Vit C degree and the influence of curcumin to the the lipied peroxidation induced by Fe2+-Vit C by the method of TBA to determinate malondialdehyde (MDA) content of liver in vitro. Results: Curcumin has stronger ability to resist reactive oxygen species in vitro and inhibit the hemolysis of red blood cells in mice induced by H2O2 oxidation, and can also inhibit the liver mitochondria swelling induced by Fe2+-Vit C, and can reduce the content of MDA. Conclusion: Curcumin has stronger anti-oxidative activity in vitro. KEY WORDS: curcumin; anti-oxidative 姜黄素体外抗氧化活性的研究 前 言 姜黄素(curcumin)化学名为 1，7-二( 4-羟基-3-甲氧基苯基) -1，6-庚二烯-3，5