五种荨麻属植物提取物抑制前列腺增生作用的研究.docVIP

Effects of 5 Urtica plants on Benign Prostatic Hyperplasia Baomin Feng* Liying Shi Ling Tang Yongqi Wang College of Bioengineering, Dalian University, Dalian, 116622, China 五种荨麻属植物提取物抑制前列腺增生作用的研究 冯宝民* 史丽颖 唐 玲 王永奇 大连大学生物工程学院，大连市经济技术开发区，116622 摘要：目的： 对5种荨麻属植物(裂叶荨麻、三角叶荨麻、滇藏荨麻、狭叶荨麻、宽叶荨麻)的地上和地下部分的20%乙醇提取物进行抑制前列腺增生作用的筛选。方法：采用去势大鼠肌肉注射丙酸睾酮注射液复制了大鼠前列腺增生病理模型，造模动物灌胃给予受试药物，连续24天，剖取前列腺称重并进行病理学检查，镜下观察前列腺中卵磷脂小体密度，同时取血清检测酸性磷酸酶含量。结果：这5种荨麻属植物的地上和地下部分的提取物大多对模型动物前列腺增生表现抑制出抑制作用；其中裂叶荨麻地上和地下部分、滇藏荨麻的地上部分、狭叶荨麻的地下部分以及宽叶荨麻的地上部分提取物可明显增加前列腺中卵磷脂小体密度；对血清中酸性磷酸酶含量影响各组同模型组比较均无统计学意义，但其中裂叶荨麻地上和地下部分、滇藏荨麻的地上部分提取物有一定减少趋势（t1.6）。组织病理学检查发现，裂叶荨麻地上部分、滇藏荨麻的地下部分、狭叶荨麻的地上和地下部分以及宽叶荨麻的地上部分对病理改变程度有减轻趋势。结论：综合各项指标发现，裂叶荨麻和滇藏荨麻的地上部分作用最强，有望开发成为抗前列腺增生的药物。 关键词：荨麻属植物，抗前列腺增生，酸性磷酸酶 Abstract Objective: To study the effects of 20% ethanol extracts of aerial and underground parts of 5 Urtica plants [Urtica fissa E. Pritz, Urtica triangularis Hand-Mass, Urtica mairei Levl, Urtica angustifolia Fisch. Ex Hornem and Urtica laetevirens Maxim subsp. dentate (Hand-Mazz.) C. J. Chen. Urticaceae] against benign prostatic hyperplasia (BPH) were observed in this paper. Method： Each extract was administrated orally for 24 days continuously after the establishment of the rats experimental prostatic hyperplasia model which was induced by the injection of testosterone propionate in castrated rats. Prostates of rats were bisected and weighed and the density of lecithin corpuscle was observed under microscope. Sera of rats were prepared and the contents of acid phosphatase (ACP) in which were detected. Results and discussion: Most extracts of Urtica plants have inhibitory activities against BPH in different degrees. Particularly, extracts of aerial part of Urtica fissa E. Pritz and Urtica mairei Levl were the most effective and might be used as agents in new drugs for men with mild to moderate BPH. This paper may be the first documentation of these 5 urtica plants in vivo against BPH activity. Key words: Urtica plants, benign pr