生理所黄阿敏优秀培训书.pptVIP

Southern, Northern and Western blotting 生理所 黃阿敏 Comparison of Southern, Northern, and Western analyses of Gene X Southern hybridization First described by E. M. Southern in 1975. Applications of Southern hybridization RFLP’s, VNTR’s and DNA fingerprinting Checking of the gene knockout mice The flow chart of Southern hybridization Southern hybridization Detection of an RFLP by Southern blotting Detection of the sickle-cell globin gene by Southern blotting Checking of the gene knockout mice Flow chart of Southern hybridization Preparing the samples and running the gel Southern transfer Probe