流式细胞仪原理及其在CD4检测中的的应用.pptVIP

* This procedure applies to the clinical laboratory environment with the BD FACSCount flow cytometer for the purpose of CD4 lymphocyte enumeration using whole blood specimens. We will use this procedure during the laboratory exercise Prime the system Disconnect both sheath tanlk and waste tank by removing quik-disconnects and sensors for both tanks. Empy the waste, add 200 ml hypochlorite Fill the sheath tank with sheath fluid up to one inch from the top. Replace reserviors, fluidic tubing and sensors. Check for bubbles in both the fluid filter and in the flow cell. Enter control reagent lot