高婷婷(国际会议英文演讲)终稿.ppt

Therefore, a phytochemical investigation（植物化学研究） on the saponin constituents of the dry bulbs of F.pallidiflora was carried out. The structures of the isolated compounds were elucidated by UV(紫外), IR（红外）1H NMR and 13C NMR（核磁共振） analysis. Plant material The dry bulbs of F. pallidiflora Schrenk (2.0 kg) were collected in Yining Xinjiang Province of China in June 2008, and identified by Dr Yong Tan of Shihezi University. Main Equipments HPLC(高效液相色谱仪 ):Waters 2695 Separations NMR(核磁共振光谱仪) :BRUKER-APX-600 Rotary evaporation(旋转蒸发仪):EYBLA Recirculated water (循环水式真空泵 ): SHZ-D9Ⅲ Methods Extraction（提取） Isolation (分离) Technology roadmap Results Three new steroidal saponins, pallidiflosides A (1), B (2), and C (3) were obtained by spectroscopic techniques and chemical means. Compound 1 26-O-β-D-glucopyranosyl-(25R)-furost-5,20(22)-dien-3β,26-diol-3-O-β-D-xylopyranosyl(1 → 4)-[α-L-rhamnopyranosyl(1 → 2)]-β-D-glucopyranoside (1) Discussion Compound 1 was obtained as a white amorphous solid with [α20D] -56.1 (MeOH, c0.052) and gave positive results with Liebermann–Burchard and Ehrlich reagent tests. The IR spectrum showed absorption bands for hydroxyl (3364 cm-1) and a glycosidic linkage (1000–1100 cm-1). 1HNMR spectrum (氢谱) the sugar moities（糖部分） 13C NMR spectrum (碳谱) The above evidences indicated that 1 was a furostanol saponin（呋喃甾烷型皂甙 ）with four sugar units. Compound 2 26-O-β-D-glucopyranosyl-3β,26-dihydroxyl-20,22-seco-25(R)-furost-5-en-20,22-dione-3-O-α-L-rhamnopyranosyl(1 → 2)-β-D-glucopyranoside Compound 2 was obtained as a white amorphous solid with [α20D] -59.2 (MeOH, c 0.052) and it gave positive results with Liebermann–Burchard and positive Ehrlich reagent tests. The IR spectrum showed absorption bands for hydroxyl (3363 cm-1), carbonyl of ester (1745 cm-1), and carbonyl of ketone (1713 cm-1). All this indicated that 2 was a furostanol saponi