3.2原核生物的转录调控toupload2016年春详解.pptVIP

Lac操纵子的O1 回文结构 In Jacob and Monad’s model, the lac operon contained a single operator (O1).??However, two additional operators (O2 and O3) were subsequently discovered. In fact, there are three operators, the classical operator O1（major operator, 主操纵基因）, centered at position +11, the downstream auxiliary operator（辅助操纵基因） O2, centered at position +412, and upstream auxiliary operator O3, centered at –82. CAP is a positive regulator of the lac operon. 四聚体LacI蛋白和DNA的结合 1、四聚体LacI蛋白的两个单体通过DNA结合结构域与Lac操纵子的O1区（中心位于+11）紧密结合，结合位点为反向重复序列。 2、另一个二聚体的DNA结合结构域与O2（中心位于+412bp处）或O3（中心位于-82bp处）疏松结合，将DNA拉