杨树根伸长区铝毒指标性超微结构的研究-应用与环境生物学报.docVIP

铝毒影响杨树根质体超微形态的研究( 吴文杰1 钱莲文2** （1.泉州师范学院 化学与生命科学学院 2.泉州师范学院资源与环境科学学院 泉州 362000） 摘 要 为研究杨树根伸长区细胞内质体铝中毒敏感性的超微形态特征，以常绿杨树（Populus×euramericana cv.A-61/186）根尖为材料，用铝浓度分别为0.074、0.222、0.370、0.518、0.666mmol L – 1水培杨树扦插苗30d、60d、90d，利用透射电子显微技术进行观察，结果显示，铝浓度0.518 mmol L–1胁迫时间30-60d的中毒结构：质体肿胀且着色深，呈大型“黑碟盘”（Black disc plate）状。质体敏感中毒铝浓度比细胞壁高。本研究首次发现并命名的“黑碟盘”（Black disc plate）质体敏感铝毒超微形态名词，作为耐铝毒细胞器鉴定指标的参考，有待在不同的耐铝杨树品种上进一步的验证，以期为今后的耐铝杨树品种研究提供基础资料。 关键词 常绿杨， 铝胁迫，质体，超微结构， 黑碟盘 Effects of Aluminum Stress on Ultrastructure of Root Plastid in Evergreen Poplar WU Wenjie QIAN Lianwen (1.College of Chemistry and Life science,Quanzhou Normal university;2.College of Resources and Environment Science, Quanzhou NormalUniversity, Quanzhou, Fujian 362000, China; ) Abstract: Ultrastructures of root plastid in evergreen poplar (Populus×euramericana cv.A-61/186) were investigated to illustrate the effects of aluminum stress on the root plasmids of elongation zone, And the aluminum stress concentration respectively are 0.074、0.222、0.370、0.518、0.666 mmol L–1 with hydroponics. The ultrastructures of root plastid were observed by using transmission electron microscopy on the 30d,60d and 90d respectively. The results indicated that obvious damage to the root Plastid is caused under aluminum stress, in which the concentration of the aluminum stress and stress time were negatively correlated. Highly acute to neutral(0.518 mmol L– 1 30-60d）poisoning structure：Plastid takes the shape of large “Black disc plate”. Plastid sensitive to toxic aluminum concentrations was higher than the cell wall. Plastid ultrastrucural sensitive to aluminum toxicity were discovered and named as “Black disc plate” for the first time in this study, Which Can maybe used as reference of appraisal indicators of organelles at tolerance aluminum toxicity,But this need to be validated in different aluminum resistant poplar varieties. The results could provide basic information for future study of aluminum -resistant varieties of poplar. Ke