大肠杆菌培养基的优化及发酵罐操作.pptVIP

大肠杆菌培养基的优化及摇瓶种子制备 目录 LB培养基配方 优化接种量 操作过程及注意事项 将配置好的种子瓶放置在摇床上培养12至24小时，设置摇床转速为180r/min，温度为37摄氏度 注意：在放置时，不要将摇瓶卡的太紧，防止摇瓶在摇床运转过程中破裂。 操作中得失误 LB培养基的制备 配置300ml培养基 Nacl 3g 蛋白胨 1.5g 酵母浸粉 3g NaOH调PH为7.4 高压灭菌 盛有250ml培养基的锥形瓶 盛有40ml培养基的锥形瓶 10支试管：两支盛有5ml培养基的试管、一支盛有10ml蒸馏水、七支盛有9ml蒸馏水，塞好棉塞。 7支1ml的移液管 14个平板 7个涂布器 全部用报纸包好放入高压蒸汽锅中灭菌30min 分离单菌落 将制作好的平板放入恒温箱中培养24至48小时，温度控制在37摄氏度。 观察结果 种子制备 挑取单菌落至种子瓶 放入摇床上培养12小时，设置温度为37摄氏度，转速为180r/min。 操作中得失误 谢谢观赏 * * 第五组 小组长：陈晓 小组成员：张立燕 王竞竞 王怡丹 支甜甜 张静 王健 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. LB培养基配方 优化接种量 操作过程及注意事项 操作中的失误 观察结果及分析 LB培养基的制备 分离单菌落 观察结果 种子制备 操作中得失误 培养基的优化 摇瓶种子的制备 生长曲线 制作详细过程 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 培养基的优化 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 10g/L 5g/L Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 10g/L 加入2%的琼脂 根据需要 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 1% 2% 4% 6% Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 接种量 1% 2% 4% 8% 菌液体积 0.4ml 0.8ml 1.6ml 3.2ml 准备四个250mL的锥形瓶，分别加入40mL的蒸馏水、0.4gNacl、0.2g酵母浸粉、0.4g蛋白胨。用NaOH调PH为7.4 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 用100ml的锥形瓶盛装种子液 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 摇瓶种子制备 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client P