葡聚糖的产品化.pptVIP

Company Logo LOGO 葡聚糖的产品化 Company Logo * 1.Introduction 【标志性成分及含量】每100克含：粗多糖48.1克、维生素C 7.9克 推荐服用人群：免疫力低下者、受辐射危害者、便秘患者、心血管病人。辅助放化疗，有一定的同步减毒增效的作用。 27g 成本价：13g，0.8元，得率按11%计算，27g的成本价为15.1元 Company Logo * 很有研究价值 Company Logo * Biological activity of the polysaccharide glucan 1.anti - tumor 葡聚糖抗肿瘤效用是刺激免疫系统的结果，而不是直接对肿瘤的毒性细胞产生作用。这种效用相对于传统治疗的优势在于，免疫细胞具有识别和摧毁“坏”细胞和“外来入侵者”的能力，所以葡聚糖对健康组织和细胞的危害很小。 2.improve function of immunological competence ;anti-virus 20世纪80年代，哈佛大学的研究陆续表明，β-1，3-D-葡聚糖可帮助人们抵抗多种病原菌的侵害，如金色葡萄球菌 3.Reduce the radiation damage 美国空军辐射生物学研究所的研究表明，酵母葡聚糖 可以很好地帮助辐射后免疫系统的恢复和抵抗能力的快速提高。 在给予致死剂量的辐射后，对照组的小鼠在2周后全部死亡， 而服用酵母葡聚糖的小鼠的死亡率只有40％。 Company Logo * Immune activator Company Logo * 1.Introduction β（1→3）糖苷键连接主链，侧链是β（1→6）糖苷键连接至少4个葡萄糖残基。 G Guizand等人用X射线法研究证明了酵母不溶性葡聚糖是以三股螺旋存在的，三条多糖链平行排列缠绕，通过链间氢键作用处于稳定状态。 约有1500个β-（1→3）-葡萄糖残基，和190个β-（1→6）葡萄糖单位 Company Logo * the cell wall of Saccharomyces cerevisiae represents up to 20% of the cell dry weight. Around 60% of this total corresponds to the β-glucans. Klis, Mol, Hellingwerf, Brul, 2002 Company Logo * 1.Introduction acid and alkaline washings lead to degradation of glucose chains extraction with hot water and enzyme an additional high-pressure homogenization step to aid in disrupting the yeast cell wall satisfactory yields preservation of the glucose chains of the polymer. Liu, Wang, Cui, Liu, 2008). Freimund et al. (2003) Liu et al. (2008) Company Logo * insolubility in water derivatization of the glucan molecule to carboxymethyl-glucan (CM-G). Slamenová et al.,2003 Company Logo * 2. Experimental Procedures Company Logo * 2. Experimental Procedures 2.1.Autolysis and hot water treatment Company Logo * 2. Experimental Procedures 2.2. Sonication tests were conducted at 20 kHz and 150W in an ice bath via a 32 full factorial design, with three replicates of the central point. The times studied, in minutes, were 2, 4 and 6 for cell dilutions of 10%, 15% and 20% in distilled water,respect