赖氨大黄酸对快速老化小鼠samp10肾组织tnf-α、il-6、nf-κb表达的影响.docVIP

赖氨大黄酸对快速老化小鼠SAMP 10肾组织COL1A1、COL3A1和COL4A2表达的影响 甄永占，胡 刚，章广玲1，1，李 冉1，王梅梅1，林雅军（1.河北联合大学基础医学院组织学与胚胎学教研室，河北 唐山063000； .卫生部北京医院卫生部北京老年医学研究所 北京 100730） 摘 要 目的 研究赖氨大黄酸（RHL）对快速老化小鼠 (senescence accelerated mouse prone 10, SAMP 10)肾组织Ⅰ型胶原α1链（COL1A1）、Ⅲ型胶原α1链（COL3A1）和Ⅳ型胶原α2链（COL4A2）蛋白表达调控的影响及其意义。方法 选取7月龄SAMP 10小鼠18只，并随机分为空白对照组、不同剂量的RHL组，另选抗快速老化小鼠 (senescence accelerated mouse resistance 1, SAMR 1) 6只作为青年对照，给药时间6周。采用hematoxylin-eosin（HE）染色观察肾脏组织病理改变，massion 染色观察肾脏组织纤维化改变， Western blotting 方法检测肾组织COL1A1、COL3A1COL4A2和NF-κB蛋白的表达。结果 SAMP 10小鼠肾组织有节段性肾小球萎缩、硬化和明显的间质纤维化，SAMR 1组和RHL组小鼠则无肾小球萎缩、硬化，RHL降低了肾组织间质纤维化，并抑制肾组织COL1A1COL3A1和NF-κB蛋白表达(P0.05)。结论 RHL可抑制SAMP 10小鼠肾组织纤维化程度，这可能是其发挥肾保护作用，从而起到抗衰老作用的机制之一。 【关键词】 赖氨大黄酸；SAMP 10快速老化小鼠；肾间质纤维化；抗衰老 【中图分类号】 R339 【文献标志码】 A Effect of rhein lysinate on the expression of COL1A1, COL3A1, and COL4A2 in the kidney tissue of SAMP 10 mice Z Yongzhan1, Hu Gang2, Zhang Guangling1, Wei Jingbo1, Li Ran1, Wang Meimei1, Lin Ya-jun2 (1.Department of Histology and Embryology, College of Basic Medical, Hebei United University, Tangshan 063000, China; 2. The key Laboratory of Geriatrics, Beijing Hospital Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China) [Abstract] Objective To investigate the effect of rhein lysinate (RHL) on the expression of COL1A1, COL3A1, and COL4A2 in the kidney tissue of senescence accelerated mouse prone 10 (SAMP 10) mice and to provide theoretical foundation for clinical research of RHL against aging. Methods Eighteen male SAMP 10 mice were selected as the research object, and were randomly divided into various groups: blank control group, and different concentrations of RHL groups, and six senescence accelerated mouse resistance 1 (SAMR 1) as young control group. After 6 weeks treatment, HE staining was used to detect the pathological change of kidney, massion staining was used to detect the fibrosis change of kidney. The expression levels of protein including COL1A1, COL3A1, COL4A2 and NF-κB were detect