1实验大论文毛雨.doc

毕 业 设 计（论文） 2014届 题 目湖州铁线莲多糖传统 提取工艺研究 专 业 制药工程 学生姓名 毛雨 学 号 指导老师 张忠山 论文字数 9412 完成时间 2013年11月 湖 州 师 范 学 院 教 务 处 印 制 湖州铁线莲多糖传统提取工艺研究毛雨 （湖州师范学院生命科学学院 湖州 313000） 摘要：实验通过单因素和正交设计法，来研究湖州铁线莲多糖提取工艺。采用传统的水提醇沉法提取湖州铁线莲多糖，并使用紫外分光光度计测定吸光度，从而测得湖州铁线莲多糖含量。所测得的吸光度值越大，则其相应的提取率也就越大。在本实验中，就把测得的吸光度值作为湖州铁线莲多糖提取率的评价指标。结果得到其最佳提取条件为：浸提温度90℃，浸提时间5h，料液比1:40。并且，根据正交实验，可知浸提温度对湖州铁线莲的多糖提取率影响最大，而浸提时间和料液比对其的影响则依次减弱。 关键词：湖州铁线莲，多糖，提取 The traditional extraction technique of polysaccharide from Clematis huchouensis Tamura MAO Yu （School of Life Science, Huzhou Teachers College, Huzhou 313000）Abstract: By single factor and orthogonal design method, to study the extraction process of Polysaccharide from Clematis huchouensis Tamura. The traditional water extraction and alcohol extraction of Clematis huchouensis Tamura polysaccharide precipitation, and spectrophotometer absorbance was measured using UV, which measured the content of polysaccharide of Clematis huchouensis Tamura. The absorbance of the measured value is greater, the extraction rate is bigger also. In this experiment, the measured absorbance value as Clematis huchouensis Tamura polysaccharide extraction rate as evaluation index. Results the optimum extraction conditions were: extraction temperature 90 ℃, extraction time 5h, ratio of solid to liquid 1:40. And, according to the orthogonal experiment, the extraction of polysaccharide of temperature on extraction rate of Clematis huchouensis Tamura the greatest impact, and extraction time on the impact of relative to some of the weak, and solid to liquid ratio on the extraction rate of polysaccharide effect is very weak. Key words: Clematis huchouensis Tamura, Polysaccharide, Extraction rate 目 录 1．引言 1 1.1 湖州铁线莲简介 1 1.2 植物多糖的传统提取工艺介绍 1 1.3 本研究的意义 2 2．材料与