正交试验优选仙茅酚苷类成分的提取方法.docVIP

正交试验优选仙茅酚苷类成分的提取方法 刘雷1,2 韩婷2薛黎明2 秦路平2 辛海量3张巧艳1,2* 1．福建中医药大学药学院，福州 350108； 2．中国人民解放军第二军医大学药学院，上海 200433 3．中国人民解放军第二军医大学中医系，上海 200433 基金项目：上海市基础研究重点项目09JC1417600；国家自然科学基金资助项作者简介：刘雷，男，1984，硕士研究生，E-mail:liulei3123@163.com *通讯作者( Corresponding author):张巧艳，电话E-mail: zqy1965@163.com [摘要] 目的：研究仙茅酚苷类成分的最佳提取方法。方法：以苔黑酚葡萄糖苷，仙茅苷，仙茅素3种酚苷类成分的含量为考察指标，以HPLC进行含量测定，采用正交试验确定仙茅酚苷类成分的最佳提取方法。结果：乙醇浓度、提取时间、提取次数和溶剂用量显著影响仙茅酚苷类成分的提取效率，其中以乙醇浓度的影响最为显著。最佳提取方法为10倍量70%乙醇回流提取3次，每次120分钟。结论：采用本研究方法能够充分提取仙茅的酚苷类化学成分。本研究对于仙茅的开发利用具有重要意义。 [关键词] 仙茅；酚苷；正交试验；提取 [中图分类号] [文献标志码] [文章编号] Optimization of extraction process for phenolic glycosides in Rhizoma Curculigins LIU Lei1,2, HAN Ting2, XUE Li-ming2, QIN Lu-ping2, XIN Hai-liang3 , ZHANG Qiao-yan1,2* 1.Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China 2.Department of Pharmacy, Second Military Medical University, Shanghai 200433, China 3.Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China [Abstract] Aim: To optimize the extraction process for phenolic glycosides in Rhizoma Curculigins. Methods: The contents of orcinol glucoside，curculigoside and curculigine A weredetermined by HPLC, the extraction conditions of phenolic glycosides in Rhizoma Curculigins were optimized by orthogonal design. Results: The concentration of ethanol, extraction time, times of extracttion and solvent volume signifficantly affected the extraction efficacy of phenolic glycosides in Rhizoma Curculigins, and effects of the ethanol concentration was most significant among the four factors. The optimal extraction process was refluxing twice with 70% ethanol (×10 times) for 120 min. Conclusion: The phenolic glycosides in Rhizoma Curculigins could be adequately extracted by the optimal extraction process, and this study has important significance for the development and explore of the phenolic glycosides in Rhizoma Curculi