一株产胶原蛋白酶芽苞杆菌的分离及鉴定.doc

PAGE PAGE 1 一株产胶原蛋白酶芽孢杆菌的分离及鉴定 生物科学2003级 李军 指导老师 吴 琦 副教授 摘 要：从皮革厂、畜肉市场、屠宰场等处采样，分离得到49株明胶水解活性的细菌。经活性平板初筛、牛皮消化实验和活性测定，筛选得到一株胶原蛋白酶活性为35.97U/mL的菌株。经形态学观察、生理生化特性和16SrDNA序列分析，鉴定该菌株为短小芽孢杆菌。 关键词：胶原蛋白酶，短小芽孢杆菌，分离，鉴定 Isolation and Identification of a Bacillus pumilus with Collagenase Activity LI Jun Biological Science, Grade 2003 Directed by WU Qi (Associate Prof. ) Abstract：Forty-nine bacterial strains with glutin hydrolyzation activity were isolated from leather house, market and slaughterhouse. By screening media, fresh calfskin decomposition and activity detection, one strain