直立密穗基因dep21388的遗传分析及在杂交稻中的育种利用论文.doc

直立密穗基因DEP2-1388的遗传分析及在杂交稻中的育种利用 胡运高1,2，郭连安2，杨国涛2，钦鹏1，范存留2，彭友林2，严维3，何航3，李仕贵1＊ 1.四川农业大学水稻研究所，成都 611130； 2.西南科技大学水稻研究所，绵阳 621010； 3.北京大学生命科学院，北京 100871摘要：通过EMS诱变恢复系蜀恢498，获得直立穗突变体1338。与野生型相比，突变体穗抗弯曲力极显著增遗传分析表明该直立穗表型受一对性核基因控制方法定位DEP2第7外显子一个A到G的，导致第928个精氨酸（AGG）被置换成甘氨酸（GGG），性状可能用1338、野生型与不育系配组，1338与弯曲穗不育系所配组合穗部表现半直立，且保持较高的结实率和杂种优势直立穗突变体1338在杂交水稻育种中的利用价值。 关键词：水稻基因定位1,2, Lianan Guo2, Guotao Yang2, Peng Qin1, Cunliu Fan2, Youlin Peng2，Yan Wei3, Hang He 3, Shigui Li1 1. Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China; 2. Rice Research Institute of Southwest University of Science and Technology, Mianyang 621010, China 3. College of Life Sciences, Peking University, Beijing 100871, China Abstracts: Using ethyl methanesulfonate (EMS) mutagenesis, we isolated an erect panicle mutant, R1338, from indica heavy-panicle restorer Shuhui498. Compared with wild type control, the mutant displayed dwarfism, erect and short panicle, short primary panicle branch, increased grain density, short grain length and increased grain thickness. In addition, the erect panicle architecture of R1388 resulted in significant decreased bending moment and increased resistance to panicle bending. Histocytological analysis indicated that the diameter of uppermost internode, cellulose content and lignin content play important roles in resistance to panicle bending. Genetic analysis revealed that the mutant phenotype was controlled by a semi-dominant nuclear gene. With resequencing and MutMap analysis strategy, we found that one SNP from A to G at the seventh exon of DEP2 resulted in the 928th amino acid substitution from arginine (AGG) to glycine (GGG) in R1338 mutant. Considering the phenotype of other dep2 mutants, the phenotype of R1338 was likely to be caused by the SNP in DEP2. The mutant R1338 and wild type were crossed with several sterile lines which respectively had different panicle types, the combinations generated from R1338 and curve panicle sterile lines showed