分子生物学技术9幻灯片.pptVIP

类型 核酸 探针 应用 Southern DNA DNA or RNA 基因拷贝数等 Northern RNA DNA or RNA RNA 大小和表达丰度 Western Protein 抗体 蛋白的大小和丰度 Comparison of Southern, Northern and Western bolt hybridization Southern and Northern blotting DNA on blot RNA on blot Genomic DNA preparation RNA preparation Restriction digestion - Denature with alkali - Agarose gel electrophoresis ? DNA blotting/transfer and fixation RNA 6. Probe labeling ? 6. Hybridization (temperature) ? 7. Signal detection (X-r