Isolation Technique Bakersfield College隔离技术贝克斯菲尔德学院.pptVIP

Isolation Technique In nature microbial cultures are mixed Identification relies upon isolating individual colonies Testing requires pure cultures As a result isolation technique provides an essential microbiological tool Mixed Culture from Raw Poultry Streak Plate Isolation Principle An original inoculum containing a mixture of bacteria is spread into 4 quadrants on solid media. The goal is to reduce the number of bacteria in each subsequent quadrant. Colonies are masses of offspring from an individual cell therefore streaking attempts to separate individual cells. Discrete colonies form as t