Statistical Considerations for Defining Cut Points and 定义切入点的统计考虑.pptVIP

* * * * * * * * * * * Ratio cutoff is 98% of Diluent, so Diluent alone and highly diluted samples tend to be positive! The floating an ratio cutoffs are better than the fixed cutoff, which ignores the diluent control. But, the ratio cutoff overcorrects for plates so instead use a partially corrected floating cutoff based on the slope. * The titer is the highest concentration with a ratio below the cut point * 74 titration curves on 24 plates of 2 species analyzed Ratios 4 excluded because more variable and to avoid prozone effects A common slope was assumed on each plate yielding 1 estimate