Vesicle Formation at the Plasma Membrane and TransGolgi质膜和反式高尔基囊泡形成.pptVIP

Vesicle Formation at the Plasma Membrane and Trans-Golgi Network: The Same but Different M. A. McNiven, et al., Science 313, 1591 (2006) Department of Biochemistry and Molecular Biology and the Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic College of Medicine, Rochester Abbreviations PM: Plasma Membrane TGN: Trans-Golgi Network CCV: Clathrin-Coated Vesicle APs: Adaptor Proteins EGF: Epidermal Growth Receptor GGAs: Golgi-localized, γ-earcontaining, Adenosine diphosphate Ribosylation Factor (ARF) PIP2: PhosphatidylInositol 4,5-bisPhosphate PI4P: PhosphatidylInositol 4-monoPhosphate UIMs: Ubiquitin Interacting Motifs Overview: yin yang relationship Sorting, packaging, vesiculation, transport of membrane and protein cargo active exchange of membranes protein cargo; highly conserved mechanisms in vesicle formation scission In and out of the cell Membrane continuum Index I. similarities and variations in sorting mechanisms II. Lipid-membrane binding, bending and pinching III. Vesicle scission I. similarities and variations in sorting mechanisms Similarities in protein-sorting mechanisms at the PM and TGN Variations of a common sorting machine 1.Similarities in protein-sorting mechanisms at the PM and TGN Key functions: selective sequestration and packaging of protein cargo Central scaffold: clathrin and its associated coat proteins APs and monomeric clathrin-associated sorting proteins “first line” of cargo-sequestration specificity AP1-4 similar organization, subunits, structure; different cellular localization patterns, vesicle-formation processes AP: mediate protein sorting Recognition of sorting motifs of the cytosolic domains of transmembrane proteins Three classes Asn-Pro-X-Tyr: AP-2 at the PM while interact with non-AP-2 Tyr-X-X-Φ: μsubunit of all 4 APs [Asp-Glu] X-X-X-Leu [Leu-Ile]: AP1-3 yet different preferences Conclusion for PART 1 Differe