HeparinControlledStimulationofinvivoAngiogenesis.pptVIP

Heparin Controlled Stimulation of in vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels Peter Fuegy Dr. Robert Peattie Celeste Riley, Dan Pike, Kyle Pomraning Dept. of Chemical Engineering Background Tissue Engineering Surgical Implants Why Are They Unsuccessful? Terms to know HA – Hyaluronic Acid PEGDA – polyethylene glycol diacrylate Terms to know VEGF – Vascular Endothelial Growth Factor Ang-1 – Angiopoietin-1 Hydrogels Heparin (Hp) Role: Glycosaminoglycan in the extracellular matrix Regulates thrombosis blood vessel formation Anti-tumor agent Why Hp? Hp-binding growth factors Chemically modified Hp-DTPH Goal The project goal is to determine whether the co-delivery of the growth factors VEGF and Ang-1 in HA/Hp-hydrogel films can induce a controlled angiogenic response in vivo Angiogenesis Angiogenesis is the process by which new capillary vessels grow and develop from preexisting vessels It can be stimulated via more than 24 GF’s as well as low molecular weight HA Previous Research The Peattie Laboratory has shown that: HA films are capable of storing and releasing GF’s over a prolonged period of time HA films produce a strong angiogenic response in vivo With Hp present HA films have been shown to regulate the release of GF’s in vitro. Summer Outline Experimental Control Setup Making HA-Hydrogels Step 1: Chemically modified HA and Hp are dissolved into PBS pH 7.4 GF’s are dissolved in as needed Making HA-Hydrogels Step 2: Crosslink HA/HP and GF’s with PEGDA Making HA-Hydrogels Here is the process of crosslinking After a period of 24 hours the gels are fully set and ready to undergo dehydration and form the film used for implantation Surgical Procedures The HA/Hp-gel implants will be evaluated in vivo in male Balb/C mice between 6 and 8 weeks of age Surgical Procedures The mice are fully anesthetized and a 4-5mm incision is made into the right ear pinna posterior surface A po