胃癌和胃溃疡组织中ERβ与hPOT1基因单核苷酸多态性相关性(崔)详解.doc

我的意见：乱！没有条理，不如拆开了，写成两篇，一篇写雌激素受体，一篇写Hp0T1。 胃癌和胃溃疡组织中ERβ与hPOT1基因单核苷酸多态性相关性 钱春野1杨留才1 周娟1 宋署2 1盐城卫生职业技术学院 2盐城市第一人民医院（江苏 盐城224006） *[基金项目]2008年盐城市医学科技发展计划项目（项目编号2008121） [摘要] 目的：研究胃癌和胃溃疡组织中ERβ与hPOT1基因单核苷酸多态性相关性。 方法：以100例原发性胃癌为实验组，90例胃溃疡为对照组，免疫组织化学SP法检测ER-(和hPOT1蛋白，RT-PCR法检测ERβCA重复序列多态性和G1082A多态性以及hPOT1基因单核苷酸多态性。 结果： ER-(在实验组和对照组的阳性表达率依次为68.0%和23.3%，而POT1的阳性率为%和1.1%,，差异G1082A 基因型与肿瘤的临床分期、分化程度、浸润深度、转移情况、五年生存情况密切相关（P0.05）,与初复发肿瘤、患者年龄、肿瘤大小、组织学类型无关（P0.05）；Genotype in hPOT1 IVS13-98G/T与肿瘤的临床分期、分化程度、浸润深度、转移情况、五年生存情况密切相关（P0.05）,与初复发肿瘤、患者年龄、肿瘤大小、组织学类型无关（P0.05）；而ERβ的CA repeat polymorphism与胃癌的所有临床特征无关。等位基因频率中仅a/A与肿瘤的分化程度、浸润深度密切相关（P0.01），其余均与肿瘤无关。 结论：ERβ和POT1均与胃癌、分化程度、浸润深度、转移及五年存活率密切相关，并且ERβ的Aa、aa基因型及POT1的GG型及等位基因G/T关系极大。 [关键词] 胃癌，临床特征，ERβ，hPOT1，基因多态性 The Expression and Correlation of ER β, hPOT1 Gene in Gastric Cancer and Gastric Ulcer Tissue in eastern China Qian Chunye1 Yang Liucai1,Zhou Juan1,Song Su2 (1 Yancheng Health Vocational and Technical College; 2 Yancheng NO.1 Peoples Hospital, 224006, China) [Abstract] Objective: To explore the polymorphisms and correlation of ER β, hPOT1 gene in gastric cancer and gastric ulcer tissue. Methods: 100 cases of primary gastric cancer as the test group, 90 cases gastric ulcer as the control group, immunohistochemical SP method was used to detect ER β and hPOT1 protein, RT - PCR method to detect the polymorphisms of ER β CA repeat sequence, G1082A as well as hPOT1 genes SNPS. Results: 1. Positive rates of ER β were respectively 68.0% and 23.3% in the test group and the control group, with significant difference (P 0.01). While those of POT1 were respectively 86.0% and 1.1%, with significant difference (P 0.01); And the positive rates of the subjects with low differentiation degree, high invasion depth, lymph node metastasis and five years death respectively were higher than those with high differentiation degree, shallow invasion depth, without lymph node metastasis and five years survival, and the clinical stagesⅠ,Ⅱwere lower signif