Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut(Arachis hypogaea L.) Seeds.pdfVIP
- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut(Arachis hypogaea L.) Seeds.pdf
Availableonlineatwww.sciencedirect,corn
FunctionalAnalysisofthePhOsphOenOIpyruVateCarboxylaseontheLipid
AccumulationofPeanut(ArachishypogaeaL.)Seeds
PANLi-juan,YANGQing-li,CHIXiao-yuan,CHENMing-na,YANGZhen,CHENNa,WAN GTong,W G
Mian,HEYa-nanandYUShan-lin
ShandongPeanutResearchInstitute,Qingdao266100,P.R,China
Phosphoenolpyruvatecarboxylase(PEPC;EC4.1.1.31)catalysesphosphoenolpyruvate(PEP)toyieldoxaloacetate,which
isinvolvedinproteinbiosynthesis.Pyruvatekinase(PK;EC2.7.1.40)catalyzesPEPtoyieldpyruvate,whichisinvolved
infatt acidsynthesis.Inthisstudy,fivePEPC genes AhPEPC1,AhPEPC2 AhPEPC3,AhPEPC4,andAhPEPC5
from peanuthavebeencloned.Usingaquantitativereal—timeIU1-PCRapproach,theexpressionpatternofeachgenewas
monitoredduringtheseeddevelopmentoffourpeanutvarieties(El1,Hebeigaoyou,Naihan1,andHuayu26).Itwas
foundthatthesefivegenessharedsimilarexpressionbehaviorsoverthedevelopmentalstagesofEl1withhighexpression
levelsat30and40dafterpegging(DAP);whereasthesefivegenesshowedi~egulraexpressionpaRemsduringtheseed
developmentofHebeigaoyou.InNaihan1andHuayu26,theexpressionlevelsofthefivegenesremainedrelativelyhigh
inthefirststage.ThePEPC activitywasmonitoredduringtheseeddevelopmentoffourpeanutvarietiesandseedoil
contentwasalsocharacterizedduringwholeperiodofseeddevelopment.ThePEPCactivityfollowedtheoilaccumulation
patternduringtheearlystagesofdevelopmentbuttheyshowedasignificantlynegativecorrelationthereafter.These
resultssuggestedthatPEPCmayplayaNimportantroleinlipidaccumulationduringtheseeddevelopmentoffourpeanut
varietiestested.
Keywords:peanut rachishypogaeaL.),phosphoenolpyruvatecarboxylase,PEPCactiviyt,lipidaccumulation
syntheticcarbonmetabolism (Cholleteta1.1996;Vidal
lNTRODUCTlON andChollet1997),andinplants,isgenerallyinvolved
文档评论(0)