酶法合成中合成酶的应用_陈华磊_侯殿志_于玥_相萍萍_朱_省略_光_蓝尉冰_概述.pdf

China Food Additives 玥1 2 12 12 123 12 12 12 12 12 1. 2. 530004 530004 3. 535011 Q559 A 1006 2513 2016 06 0114 11 Research on synthase application in enzymatic synthesis 1. Engineering Center for Sugarcane and Cane sugar Guangxi University Nanning 530004 2. Engineering Center for Sugar and Comprehensive Utilization Ministry of Education Nanning 530004 3. Qinzhou University Qinzhou 535011 Enzymatic synthesis has become a hot research topic in the synthesis of high molecular weight compounds due to the advantages of efficiency of the synthesis pollution free high specificity low impurity content continuous producing and easily control of molecular weight.This study provides a more ef?cient and convenient way for the synthesis of biological macromolecules by enzymatic synthesis. The research of enzymatic synthesis is still in the exploratory stage and the reportsare few. To explore high activity and high stability of the synapses is the key in the enzyme engineeringof preparation of biological macromolecules. In this paper the types and application of enzymes the mechanism of action and the improvement of the catalytic performance as well as other aspects of domestic and foreign research status are reviewed and its application prospects and future research directions are discussed. enzymatic synthesis synthase mechanism catalytic properties 2016 03 11 1989 *2013 2013GXNSFAA019036 12 34 China Food Additives 9 39.5% Seo 10 B 512FMCM 2.5 4M TASO L.M. 5 GH70 6 Dextransucrase N C Signal peptide 32 38 Variable region 140 261 N terminal catalytic domain 900 1000 C terminal glucan binding domain 30 50kDa 500 12 Leuconostocmesenteroides 70 1250 1600 150 200kDa 7 11 11 Alternansurase 13 14 L.mesenteroides CBI 110 15 2057 C 12 17 228kDa 16 AB Rabelo 8 α Lopez 18 pH 5.4 45 30min China Food Additives pH5.2 5.4 NaAc HAc Cote 19 20 SK24.002 52.8g/L α1 4 Cambon 21 Kim 25 53 A1 443 692 A A2 829 961 B1 373 442 B B2 962 1056 C 693 828 1 244 372 2 1057 1087 N GH13 Amylosucrase 2