bFGF对全脑缺血再灌注大鼠海马CA1区内源性神经干细胞的影响.doc

bFGF对全脑缺血再灌注大鼠内源性神经干细胞的影响摘要目的 探讨bFGF对全脑缺血再灌注大鼠内源性NSCs增殖、迁移和分化的影响。方法 选用体重在250～300g的健康雄性SD大鼠，随机分为假手术组（A）、模型组（B）、bFGF治疗组（）。侧脑室插管并固定，建立给药途径；全脑缺血再灌注模型。分别于全脑缺血再灌注后3d、7d、11d、15d取脑，处死前腹腔注射BrdU标记液。HE染色观察大鼠海马神经元的形态学改变；免疫组织化学方法动态检测大鼠脑海马BrdU和Nestin的表达；利用RT-PCR技术半定量检测大鼠NSE mRNA的表达。结果（1）HE染色：模型组神经细胞稀疏细胞间隙增大并可见大量细胞变性坏死表现为胞体皱缩核固缩深染核仁消失；而组神经细胞存活数量增多，损伤程度减轻。（2）免疫组织化学假手术组大鼠海马区很少见到阳性细胞表达；手术各组大鼠脑海马区的BrdU和Nestin阳性细胞均在全脑缺血再灌注3d后开始显著增加，模型组7d达到高峰， bFGF治疗组11d达到高峰，随后开始逐渐下降；与模型组相比缺血后各时间点， bFGF治疗组BrdU和Nestin阳性细胞数明显增加P0.05）。（3）各组NSE mRNA表达11d前没有明显变化，15d表达有所增加仅bFGF治疗组增加具有统计学意义P0.05）。结论（1）全脑缺血再灌注能够引起内源性神经干细胞的原位增殖；（2）bFGF可促进并延长全脑缺血再灌注大鼠内源性神经干细胞原位增殖；促进内源性神经干细胞向神经元分化关键词 全脑缺血；内源性神经干细胞；bFGF；大鼠Effect of bFGF on Endogenous NSCs in Rats hippocampus with Global Cerebral Ischemia-reperfusion Ren Ming-xin1, Guo Yi-wei1, Feng Zhi-bo1. Department of Anatomy, Xinxiang Medical University, Xinxiang 453003, Henan Province.) Correspondence to：Feng Zhi-bo, Master, Xinxiang Medical University, Xinxiang 453003, Henan Province. 【Abstract】OBJECTIVE：Study the effect of bFGF on endogenous neural stem cell proliferation, migration and differentiation in rats hippocampus with global brain ischemia-reperfusion.Methods：Male Sprague-Dawley rats ( 250 g ~300 g ) were divided into there groups: sham operation group (A), operation group (B), bFGF group (C). The global brain ischemia-reperfusion models were established, and then the bFGF was injected into the lateral ventricle in group C. The doses of 5 μl (0.1 μg/μl) was administrated before the model operation, and once every two days after operation. The rat brains were acquired at 3rd d, 7th d, 11th d, 15th d with intraperitoneal injection of BrdU 1 d before animal sacrifice. HE and immunohistochemistry were used to observe the pathological changes and the expression of BrdU and Nestin in cerebral hippocampus and cortex. RT-PCR was used to measure the NSE mRNA in brain tissue. Results：(1) HE staining: There were a lot of degenerated and necrotic neurons and the bodies wer