新型微生物多糖PS238+发酵条件的研究.pdf

J031 Alcaligenes NX-3 , , , K2HPO4 6.5 g/L K2HPO4 2 g/L MgSO4 0.3 g/L 22.85g/L PS-238 1 PS-238 , 49 g/L , 10.78g/L 30% 50-100 pH2-13 10% 150 Alcaligenes NX-3 PS-238 PS-238 2 2.1 2.2 NX-3 Alcaligenes NX-3 121 15min 2.3 (g/L) 15min (g/L) 20 30 30 ,200r/min 16h 6 1 K2HPO4 2 MgSO4 0.1 pH7.0-7.2 8 K2HPO42 MgSO4 0.1 pH7.0-7.2 121 50mL/500mL, 1 30 ,200r/min 2.4 1 2.4.1 1mL 25 2.4.2 NDJ 1 ,4 2.4.3 pH 2.4.4 70-80 4 10% 72h 752 ,30r/min, 15min, 80mL 600nm 2 95% 95% 50 3 3.1 PS-238 500mL 1 PS-238 Table 1 Effects of various carbon sources on PS-238 synthesis Carbon source Viscosity pH Cell Growth (A600) (cp) Yield (g/L ) Glucose Sucrose Maltose Corn Flour Glycerol Lactose Citric acid Fumaric acid 3.2 PS-238 4.80 5.30 5.27 6.36 8.5 4.43 8.71 8.28 0.426 0.474 0.588 0.574 0.087 0.284 0.016 0.044 4000 3500 2200 2100 - 8.44 10.78 7.28 9.24 - * 025E-mail xuh@ (1980-) , ,2002 , 025E-mail:liser1317 eyou com 2 (NH4)2SO4 NH4Cl NH4NO3 (NH4)2HPO4 NH4Cl NH4NO3 (NH4)2SO4 , 1, 0.8% A600*25 Yield (g/L) Viscosity(cp) 12 6000 A *25 600 10 viscosity 5000 Yield 8 4000 6 3000 4 2000 2 1000 0 Yeast extract Beef extract Peptone 0 (NH4)2SO4 Urea (NH4)2HPO4 1. PS-238 Fig 1 Effects of various nitrogen sources on PS-238 synthesis 3.3 K2HPO4 MgSO4 PS-238 K Mg2+ K2HPO4 MgSO4 PS-238 K2HPO4 2 g/L, MgSO4 0.4 g/L 0.1% 0.5% 14 12000 12 8000 7500 12 10 8000 7000 10 pH A *25 600 8 6500 Yield 8 viscosity pH A600*25 4000 Yield viscosity 6000 6 6 5500 4 1 234 K HPO (g/L) 24 0 5 4 0.1 0.2 0.3 MgSO (g/L) 4 2. K2HPO4 MgSO4 PS-238 Fig 2 Effects of K2HPO4 and MgSO4 on PS-238 production 3.4 PS-238 0.4 5000 0.5 3 pH A600*25 Yield(g/L) Viscosity(cp) pH A600*25 Yield(g/L) viscosity(cp) ( 2) 4 statistica6.0 Table2.Main components of fermentation medium Factors -1.68179 -1 Levels 0 A: sucrose (g/L) 23.18210 30 40 B:peptone(g/L) 6.31821 7 8 C: K2HPO4(g/L) D: MgSO4(g/L) 1.31821 0.31821 2 1 3 2 1 50 9 4 3 1.68179 56.81790 9.68179 4.68179 3.68179 Y=18.2305+2.6525x1-0.3133x12+0.2949x2-0.7259x22-1.1144x3-1.7509x3