OCT三维组织模型中的细胞动力学.docxVIP

Optical coherence tomography of cell dynamics in three-dimensional tissue models光学相干断层扫描三维组织模型中的细胞动力学Abstract:摘要 Three-dimensional cell-based tissue models have been increasingly useful in the fields of tissue engineering, drug discovery, and cell biology.细胞单元三维组织模型在组织工程，药物研发和细胞生物学领域中越来越有用。While techniques for building these tissue models have been advanced, there have been increasing demands for imaging techniques that are capable of assessing complex dynamic three-dimensional cell behavior in real-time and at larger depths in highly-scattering scaffolds.尽管用于构建这些组织模型的技术一直在发展，但是对于成像技术能够在高度散射下实时地和成像更深地来评估细胞三维复杂动态的行为的需求不断增长。Understanding these cell behaviors requires advanced imaging tools to progress from characterizing two-dimensional cell cultures to complex, highly-scattering, thick three-dimensional tissue constructs.了解这些细胞行为需要先进的成像工具，从具有二维特征的细胞培养进展到复杂，高度散射，密集三维组织构造。Optical coherence tomography (OCT) is an emerging biomedical imaging technique that can perform cellular-resolution imaging in situ and in real-time.光学相干断层扫描（OCT）是一种新兴的生物医学成像技术，可以进行指定位置和实时的细胞分辨率成像。In this study, we demonstrate that it is possible to use OCT to evaluate dynamic cell behavior and function in a quantitative fashion in four dimensions (three-dimensional space plus time).在这项研究中，我们证明，有可能使用OCT在四个维度（三维空间加时间）来定量评估动态细胞行为和功能。We investigated and characterized in thick tissue models a variety of cell processes, such as chemotaxis migration, proliferation, de-adhesion, and cell-material interactions.在密集组织模型中我们研究和表示出了各种细胞内的过程，如趋化性迁移，增殖，解粘附和细胞 – 物质的相互作用。This optical imaging technique was developed and utilized in order to gain new insights into how chemical and/or mechanical microenvironments influence cellular dynamics in multiple dimensions.开发和使用这种光学成像技术，以获得对于化学和/或机械微环境在多维度如何影响细胞动力学的新发现。With deep imaging penetration and increased spatial and temporal resolution in three-dimensional space, OCT will be a useful tool for improving our understanding of complex biological interactions at the cellular le