White-tea-(Camellia-sinensis)-inhibits-proliferation-of-the-colon-cancer-cell.ppt

* White tea (Camellia sinensis) inhibits proliferation of the colon cancer cellline, HT-29, activates caspases and protects DNA of normal cells againstoxidative damage YinDan 2015210061 Food Chemistry Contents 1 Introduction 2 Materials and methods 3 Results and discussion 4 Conclusion part 1 : Introduction Introduction 96,830 cases of colon and 40,000 cases of rectal cancer are expected to occur in 2014 surgical resection combined with chemotherapy by cytotoxic drugs and radiation modification of signal transduction pathways cell cycle check points carcinogen activation/ detoxification scavenging of reactive oxygen species apoptosis Introduction white tea extracts the antioxidant activities on the inhibition of proliferation of the colorectal cancer cell line, HT-29 role in protecting against oxidative damage to DNA part 2 : Materials and methods Materials and methods ——methods evaluate the antioxidant activities of white tea on the inhibition of proliferation of the colorectal cancer cell line, HT-29 role in protecting against oxidative damage to DNA MTT assay； GloMax microplate luminescence reader； Fluorescence microscopic examination the comet assay; UV-fluorescence microscope FRAP, DPPH, nitricoxide, superoxide anion , hydroxyl radical scavenging assays. 2.1 Chemicals All the solvents and reagents used in the study were of analytical grade and used without any further purification. 2.2 Tea samples and preparation white tea extracted with hot water for 5 min and the water extracts were freeze–dried. Materials and methods ——materials Silver needle, KWF Food Industries, China 2.3 Cell culture Human colorectal adenocarcinoma cells (HT-29), human dermal fibroblasts-adult (HDF-a) and mouse fibroblasts (3T3-L1) 2.4 Statistical analysis Experimental results are presented as means ± SD, and all measurements and analyses were carried