黄东海表层沉积物中甾醇类生物标志物的分布--终2试编.docxVIP

黄东海表层沉积物中甾醇类生物标志物的分布研究完成日期：指导教师签字：答辩小组成员签字：黄东海表层沉积物中甾醇类生物标志物的分布研究摘要本文利用气相色谱仪测定了黄东海表层沉积物中四种甾醇类生物标志物的浓度，多种参数分析了该海域甾醇的环境指示意义。结果表明四种甾醇总浓度范围为：0.57-6.41μg/g，均值为2.51μg/g。总浓度的高值出现在钱塘江江口和象山湾湾口，单体浓度呈现出豆甾醇＜胆固醇＜β-谷甾醇＜胆甾烷醇。胆固醇在大多数站位都有较高的浓度。胆甾烷醇分布表明宁波海域＞台州海域＞舟山海域，并与区域的发展水平相吻合。研究区域只有胆甾烷醇与TOC（总有机碳）有较好的线性关系（R=0.619，P=0.057），其他的均无明显线性关系，表明物源输入是影响甾醇与TOC线性关系的重要因素。关键词：甾醇；黄东海；表层沉积物；生物标志物Study of distribution of sterols biomarkers in surface sediments from the Yellow Sea and East China SeaAbstract Four sterols biomarkers in surface sediments from Yellow Sea and East China Sea were quantified by gas chromatography, and environmental indicator means of sterols were apportioned based on a variety of analysis methods. It was found that the total concentrations of sterols varied from 0.57 to 6.41μg/g, with a mean value of 2.51μg/g.The higher value of the total concentrations of sterols appeared in Qiantang River estuary and Xiangshan Bay mouth, and the concentration of four sterol monomer was found to in increasing order as stigmasterol ＜cholesterol ＜β-sitosterol ＜cholestanol. Cholesterol in most stations have a higher concentration.The concentration of cholestanol was found to in increasing order as Ningbo ＞ Taizhou ＞ Zhoushan , and it has a good relevance to research areas economic developing levels. Only cholestanol has a positive correlation to TOC (R = 0.619 P = 0.057)in the study area for four sterols, the other three sterols have no significant correlation. It showed that sterols input was one of important factor of correlation between sterol and TOC.Keywords:the Yellow Sea and East China Sea;surface sediments; sterols; biomarkers目录1 绪论31.1生物标志物的建立和研究历史31.2甾醇类生物标志物的应用31.3沉积物中甾醇类的组成及含量的影响因素31.3.1甾醇类来源的影响因素31.3.2甾醇类降解的影响因素31.3.3甾醇类组成及含量的其它影响因素31.4甾醇类物质的形态分布31.5国内研究现状31.6本文的研究目的和意义32材料与方法32.1样品采集及区域概况32.2试剂及仪器32.2.1试剂32.2.2仪器32.3样品分析32.3.1实验前预处理32.3.2标准溶液配制32.3.3样品分析32.4仪器分析32.5质量控制（QC）与保证（QA）33 结果与讨论33.1 甾醇的浓度及分布33.2 胆固醇和胆甾烷醇的环境指示意义33.3 豆甾醇和β-谷甾醇的环境指示意义33.4 TOC与四种甾醇的线性相关性3参考文献3致谢31 绪论