萝卜D染色体在7号连锁群的定位研究.pdfVIP

蔬菜379 萝bD染色体在7号连锁群的定位研究 丁云花1 Budahn PeterkaHerbert2 Holger2 D一06484) (1北京蔬菜研究中心，北京100089；2德国栽培作物育种研究中心，Quedlinburg 摘要：在已知萝hD染色体附加系对线虫具有抗性和萝h分子图谱的7号连锁群定位 加系为材料，筛选到90个萝pD染色体的特异标记，选择4个在萝h构图亲本间表现多态性 RAPD标记一起用Join-map3．0进行连锁定位分析。结果萝hD染色体标记全部定位于7号 连锁群，并且7号连锁群上只有D染色体的特异标记，说明萝h7号连锁群就是D染色体在 分子水平的反映。证实了定位有抗线虫基因Hs产”的7号连锁群对应于具线虫抗性效应的萝 bD染色体。本结果对研究萝bD染色体控制的农艺性状或抗逆性以及萝h抗线虫育种和将 萝h抗线虫基因转入其他芸薹属作物的研究都将会起到重要的指导作用。 关键词：萝b；附加系；D染色体；连锁群 Dof sativushasbeendeterminedtobethenematodere— Abstract：ChromosomeRaphanus 7 hasbeenlocated of sativus the Hs产曲 sistance—bearing．andlinkagegroupRaphanus gene resistance carried controllingtonematode．Onthisbasis，thisworkwas outtOresearchthere- lationofchromosomeDand toseewhether 7 is the linkagegroup7 linkagegroupessentially ofchromosomesDonDNA1evel．90markers tOchromosomeDwerescreened map assigned from additionlines rape—radish byusing(dp)RAPDtechnology．4polymorphicassigned markerswerefurther in of245individuals，andthenwere mappedFzpopulationcomprising software 3．0 with505AFLPandRAPDmarkershasbeen analyzedby joinmaptogether which usedtoconstructradishmolecular aresult。allofchromosomeD markers map．As assigned into were werelocated 7，andin 7，therenomarkers to linkagegroup linkagegroup a