谷氨酸棒杆菌代谢产物尿苷二磷酸葡萄糖与尿苷二磷酸半乳糖的高效液相色谱测定方法.pdfVIP

L050 ?????????л?????????????????????????????? ????????Ч??????????? С?? ???? ????? ???? ???? ????? ????????????????????﹤????????????? 361005 ?? ? ????Zorbax NH ????? id.4.6 mm ??250 mm??5 ??m??????0.125 mol/L KH PO ?????(pH 3.6) 2 2 4 ????????????????????????????л????????????????????????????????????????? ????Ч????UDP-???????UDP-???????????????? 12.1 min ?? 12.7 min?????????????? ??????????Ч????????UDP-???????UDP-????????????????????????????????? ???????л?????а???UDP-???????UDP-??????????????????????????????? ????? ??Ч?????? ??????????????? ?????????????? ???????? ?????? SEPARATION OF INTRACELLULAR INTERMEDIATES URIDINE DIPHOSPHOGLUCOSE AND URIDINE DIPHOSPHOGALACTOSE OF CORYNEBACTERIUM GLUTAMICUM BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WU Xiaojie HE Ning LI Qingbiao DENG Xu LU Yinghua YAO Chuanyi (Depa rtment of Chemical and Biochemical Engineering,X iamen University, X iamen 361005,Fuj ian,P.R. China) Abstract Uridine diphosphoglucose (UDP-Glc) and Uridine diphosphogalactose (UDP-Gla) in the cell free extracts of Cory nebacterium glutamicum CCTCC M111 were successfully separated using Zorbax NH2 column (id.4.6 mm ??250 mm??5 ??m) with 0.125 mol/L potassium phosphate buffer(pH 3.6)-acetonitrile. The retention time of UDP-Glucose and UDP-Glactose were 12.1 minutes and 12.7 minutes respectively. With the calibration line of UDP-Glc and UDP-Gal??recovery rate and precision, HPLC was proven to be a suitable way for the assay of UDP-Gal and UDP-Glc in the cells of Cory nebacterium glutamicum CCTCC M 111. Relationship between the content of UDP-Gal, UDP-Glc and bioflocculant production was also discussed in this paper. Keywords HPLC, uridine diphosphoglucose, uridine diphophogala