DNA甲基化功能研讨.ppt

RRBS (procedure) : RRBS (assessment) : Advantages Enrichment of CpGs Low sample input Disadvantages Restriction enzyme limitation BS-seq flaws BS-seq, TAB-seq and oxBS-seq--TET oxygenases BS-seq, TAB-seq and oxBS-seq--scheme BS-seq, TAB-seq and oxBS-seq--result Bisulfite Sequencingmapping schemes sequence changes: Problems: Searching space is significantly increased (Waston Crick) vs (BSW, BSWR, BSC, BSCR) Sequence complexity is reduced C to T mapping is asymmetric (complicates the matching process) Problems: Bismark scheme: BSMAP scheme: BSMAP scheme: Thank you! For BSW and BSC, C