金樱子多糖对阿霉素的增效减毒作用.doc

金樱子多糖对阿霉素的增效减毒作用 姚 佳1 2，彭 梅1 2，杨 娟2 * （1.贵州大学 生命科学学院，贵州 贵阳 550025； 2.贵州省中国科学院天然产物化学重点实验室，贵州 贵阳 550002） 摘 要：无菌抽取培养在小鼠腹腔内生长良好的S180腹水瘤制成匀浆，右腋接种，建立小鼠S180实体瘤模型。连续给药10 d，通过计算抑瘤率q值脾脏指数胸腺指数检测白细胞（WBC）、红细胞（RBC），Elisa法测定脾脏的超氧化物歧化酶（SOD）、丙二醛（MDA），判断金樱子多糖对化疗药物的增效减毒作用。ADM单用抑瘤率为32.57%，与金樱子多糖合用后q 1.15）；ADM合用组与ADM组比较，外周血细胞（RBC、WBC）脏器指数SOD均明显增加（P 0.05），而MDA明显降低（P 0.05）金樱子多糖与化疗药物阿霉素合用后具有明显的增效减毒作用。 关键字：金樱子多糖S180；增效减毒中图分类号: 文献标识码: 文章编号: Experiments on Efficiency Enhancing and Toxicity of Rosa Laevigata Michx Polysaccharides on Adriamycin. YAO Jia1, 2 ，PENG Mei1, 2 ，YANG Juan2 * College of Life Sciences, Guizhou University, Guiyang Guizhou 550025, China. 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang Guizhou 550002, China) Abstract：Sterile extraction S180 which training in the abdominal cavityascites tumor growth and made homogenate,收稿日期：基金项目：[（黔科合SY字(2011）3022] 作者简介：（198-），女，*通讯作者：（1971-），，，，，linyj401@ A tumor mouse model was developed by right axillary vaccination 0.2 mL S180, and the mice were treated for 10 d. After 10 d consecutive administration, the inhibitory rate of tumor, q value, thymus index and spleen index, white blood cells in serum measuring were detected, Elisa for the determination of the spleen superoxide dismutase (SOD), malondialdehyde (MDA). The combined groups of RLP and ADM can enhance the anti-tumor effects (q 1.15), reduce the toxicity of adriamycin. The combined groups of high dose RLP and ADM can increase peripheral blood cell， organ index and the content of SOD (P 0.05), while reduce the content of MDA（P 0.05）. RLP has efficiency enhancing and toxicity reducing effect. Key words：Rosa Laevigata Michx PolysaccharidesS180； efficiency enhancing and toxicity 阿霉素由于其抗瘤谱广，抗瘤活性强，被广泛应用于临床。但它容易引起骨髓抑制心脏毒性消化道反应脱发等许多不良反应，长期服用还会发生脂质过氧化[1]。而多糖类成分由于具有一定抗肿瘤作用，同时相对毒性较低，被认为是较好的化疗辅助药物。该类成分可以通过增强机体的免疫力，减轻化疗药物的毒性，从而达到对化疗药物增效的作用[]。冯承恩[]等报道了金樱根多糖具有体内抗肿瘤作用，而金樱子的多糖抗肿瘤及其对化疗药物的增效减毒作用目前还未见报道。本文通过建立小鼠S18