cartilage tissue and bone tissue.ppt

Cartilage and Bone 肥大细胞 1 取小白鼠一只（脱臼处死），沿腹中线剖开腹壁。 2 剪取皮下组织少许，置于载玻片上，用针尖拨成薄片，空气中晾干。 3 95%酒精固定10分钟，水洗。 4 甲苯胺蓝染色2分钟，水洗。 5 90% 、95% 、100% 脱水3分钟，二甲苯透明2分钟，树胶封片。 观察结果 实验报告：高倍镜绘图：肥大细胞形态结构； 文字分析所得结果，说明什么问题？ * * 一、hyaline cartilage HE stain cross section of cat trachea a ring 34 Low power： Perichondrium :A layer of D.C.T Matrix---in pale blue color ; Chondrocyte—lie in martrix 1.hyaline cartilage 2.perichondrium 1 2 High power： peripheral chondrocyte =small, ovoid or round ;singly scattered ; central chondrocyte ==large ,round or ovoid; isogenous groups; lacunae-occupied by chondrocyte; cartilage capusle—the narrow bands surrounding the chondrocyte is stained strong basophilia 1.perichondrium 2.chondrocyte 3.cartilage capusle 4.matrix 5.isogenenous groups 1 2 3 4 5 Decalcified section of bone ( special stain) Low power： periosteum and endosteum are not clear . 1. External circumferential lamella：ten or more layers of parallel lamella along the surface of bone. 2.Osteon：composed of concentric lamellae with a central canal(round or ovoid and dark ) , also called Haversian system 3 .Interstitial lamella：irregular; located among osteons 4.Bone lacuna: present dark dots –occupied by the body of osteocyte in life 1 3 2 4 Section bone ：（low power） 1.osteon 2.central canal 3.perforating canal 1 2 3 4.internal circumferential lamella： several lamella surrounding the inner surface of the bone 4 High power： bone lacuna—ovoid in shape and the body osteocyte is not clear. bone canaliculus: to radiate as fine threads from lacuna occupied by processes of osteocyte in life 1.bone lacuna 2.bone canaliculus 3.central canal 1 2 3 三、section of bone： cross section of diaphysis of huamn long bone HE stain macroscoptic：irregular ring in shape with a central bone marrow Low power： Blood vessels and nerve have