betv,themajorbirchpollenallergen,andmald,themajorappleallergen,crossreactatthe.docVIP

Bet v 1, the major birch pollen allergen, and Mal d 1, the major apple allergen, cross-react at the level of allergen-specific T helper cells Journal of Allergy and Clinical Immunology - Volume 102, Issue 4 (October 1998) ?-? Copyright ? 1998 Mosby, Inc. ?-? About This Journal Remove Journals Issue Alert Add Journals Issue Alert Food and drug reactions and anaphylaxis Bet v 1, the major birch pollen allergen, and Mal d 1, the major apple allergen, cross-react at the level of allergen-specific T helper cells Ruth Fritsch MD Barbara Bohle PhD Ute Vollmann Ursula Wiedermann MD, PhD Beatrice Jahn-Schmid PhD Monika Krebitz PhD Heimo Breiteneder PhD Dietrich Kraft MD Christof Ebner MD Vienna, Austria Key words Oral allergy syndrome birch pollen apple Bet v 1 Mal d 1 IgE T helper lymphocyte From the Institute of General and Experimental Pathology, Department Immunopathology, University of Vienna, Vienna. Supported by grants SO6704-MED and SO6707 of the Fonds zur Forderung der Wissenschaftlichen Forschung, Austria.Received for publication Feb 13, 1998. revised June 11, 1998.accepted for publication June 23, 1998. Reprint requests: Christof Ebner, MD, Institute of General and Experimental Pathology, University of Vienna, Wahringer Gurtel 18-20, A-1090 Vienna, Austria. Copyright ? 1998 by Mosby, Inc. 0091-6749/98 $5.00 + 0??1/1/92706  Background: Food allergy to apple is frequent in individuals allergic to tree pollen. The major allergens of birch, Bet v 1, and apple, Mal d 1, have been cloned and sequenced and display a high degree of sequence identity, leading to IgE cross-reactivity.Objective: We sought to investigate cross-reactivity between Bet v 1 and Mal d 1 at the level of allergen-specific T lymphocytes.Methods: PBMCs of 13 patients allergic to birch pollen with oral allergy syndrome to apple were stimulated with rBet v 1 and rMal d 1, respectively, thereby establishing allergen-specific T-cell lines and T-cell clones. rMal d 1-speci