瘢痕疙瘩候选基因与染色体7p11微卫星扫描及连锁分析的多家系研究.pdfVIP

中国组织工程研究与临床康复多12 番第28 JJIJ 21∞8-07-08 出版 JouTTUJl of Clinical Rehabilitative TlSsue E暗的eering Research July 8, 2，侃归协U2， No.28 @突忘寰翩医学 癫痕疙瘩候选基因与染色体7p11微卫星扫描及连锁分析的多家系研究*食 刘晓军，高建华，宋玫，陈阳，严欣 Microsatellite scanning and linkage analysis of keloid candidate gene and cbromosome 7pll: A multi-pedigree study Liu Xiao寸un ， Gao Jian-hua, Song Mei, Chen Yang, Yan Xin Abstract AIM: For the relationship of keloid onset and relevant gene，也e present study analyzed the linkage between virulence gene of Chinese keloid pedigrees and chromosome 7pll to locate 由e site of predisposing genes. METHODS: Peripheral venous blood s缸nples of 69 members in the three keloid pedigrees were collected by microsa忧llite sc缸ming and Iinkage analysis. Four markers on chromosome 7p 11 were selected as microsatellite markers accordingω Iitera阳res. Subsequently，也ese markers were amplified by multiplex polymerase chain reaction (mPC町， and all 町R products were genoty严d. LOD scores at each marker site were calculated by MLINK program of 山恨AGEso位ware of the linkage analyses 10 judge 由eir linkage relationship. RESULTS: Among 也e 4 selec能d marker百，也e two point LOD scores were less 也an 1 at 0= 命0.5， they were excluded the linkage 10 the disease locus. CONCLUSION: Keloid susceptibility 1∞1 扭曲e Chinese keloid 严digrees were not on chromosome 7pll. dem佣stra血.g 1ωω heterogeneily in familial keloid formation. Liu XJ, Gao JH, Song M, Chen Y, Yan X. Microsatellite scanning and 1inkage analysis of keloid candida也 gene and chromosome 7p 11: A multi-pedigree sωdy.Zhongguo Zuzhi Gongcheng Yanjíu yu Linchuang Kan脾 2∞8;12(28):5453-5456(αína) ( lzglckL崎oumallupfiles/08-28/28k-5453(ps).pdη 摘要 目的g 从与癫痕疙瘩发病可能存在密切关系的基因出发，分析中国汉族癫痕疙瘩家系致病基因与染色体 7pl1区域是否存在 连锁关系，以定位易感基因位点。 方法z 采用微卫星扫描及连锁分析方法，选取 3 个中国汉族癫痕疙瘩家系中 69 名成员的外周静脉血标本，根据文献选择位 于 7pll 区域 4 个微卫星标记，应用多重聚合酶链式反应(mPCR) 扩增产物片断，测定 PCR 产物片段，获得每个样本的基 因分型。运用连锁分析软件 LINKAGE 的 MLINK 程序计算每个标记位点的 IβD 值，根据两点问 LOD 值判断是否存在连锁 关系。 结果s 在重组率(J =O句0.5 时，这些微卫星标记的两点 1βD 值绝大部分都小于 1. 排除连锁关系存在. 结论z 中