中山大学《细胞生物学》思考题 2012年1.pdfVIP

11. You are studying the binding of proteins to the cytoplasmic face of cultured neuroblastoma cells and have found a method that gives a good yield of inside-out vesicles from the plasma membrane. Unfortunately, your preparations are contaminated with variable amounts of right- side-out vesicles. Nothing you have tried avoids this variable contamination. A friend suggests that you pass your vesicles over an affinity column made of lectin coupled to solid beads. What is the point of your friends suggestion? Thinking and Testing (10-29-2012) 2解题艺术与技巧（第一题） 科学素质 实验技能 人文素质 实验分析题 题型 ●膜糖分布 ●凝集素的性质 ●质膜“破镜重圆” 知识点 正常与外翻膜泡 陷阱 蛋白质分离技术 技能 层析技术 原理及应用 知识点 层析介质的选择 陷阱 平稳心态 情商 ●不放弃 ●沟通与交流 核心点 Unfortunately 陷阱 31. Mutations in the genes encoding collagens often have detrimental consequences resulting in severely crippling diseases. Particularly devastating are mutations that change glycine residues, which are required at every third position in the protein chain so that it can assemble into the characteristic triple- helical rod. A. Would you expect that collagen mutations are detrimental if only one of the two copies of a collagen gene is defective?(10个字以内） B. A puzzling observation is that the change of a glycine residue into another amino acid is most detrimental if it occurs toward the amino terminus of the rod-forming domain. Suggest an explanation for this. (10个字以内） 45.1 BASIC CHARACTERISTICS OF CELL-SIGNALING SYSTEMS 5.1.1 细胞通讯的一般过程和反应 信号分子合成 信号分子释放 信号分子转 运寻找靶细胞 信号分子 与受体结合 启动细胞内 信号反应途径信号解除 表 面 受 体 细 胞 质 受 体 第 二 信 使 级 联 放 大 生 化 反 应 基 因 表 达 信号分子水解 受体修饰 浓度调节 神经细胞、 内分泌细胞 突触、血管 细胞外 5第二信使至少有两个特征: ●是第一信息同其膜受体 结合后最早在细胞膜内 侧或胞浆中出现，仅在 细胞内部起作用的信息 分子； ●能启动或调节细胞内稍 晚出现的反应。 细胞内主要的第二信使 有：cAMP、DAG、 IP3、cGMP和Ca2+。