人教版教学演示课件1-1DNA重组的基本工具.pptVIP

定向基因改造设想 设想一 能否让禾本科的植物也能够固定空气中的氮？ 能否让细菌“吐出”蚕丝？ 设想二 能否让微生物产生出人的胰岛素、干扰素等珍贵的药物？ 设想三 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 基因工程的别名 操作环境 操作对象 操作水平 基本过程 结果 基因拼接技术或DNA重组技术 生物体外 基因 DNA分子水平 人类需要的基因产物 剪切 → 拼接 → 导入 → 表达 什么叫基因工程？ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 基因工程需要哪些基本工具? Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 培育抗虫棉需要哪些工具？ 分子手术刀—限制性内切酶 分子缝合针—DNA连接酶 分子运输车—运载体 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 第1节DNA重组技术的基本工具 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 一、限制性核酸内切酶 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 思考 在自然界中有一些生物的DNA可能进入另一种生物的细胞中。我们有没有学过相关的实例？ 细菌为什么不会因外源DNA的入侵而灭绝？ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 识别双链DNA 分子的某种特定的核苷酸序列，并且使每一条链中特定部位的两个核苷酸之间的磷酸二酯键断开。 主要是从原核生物中分离纯化出来的一种酶。 4000种。 1、来源： 2、种类： 3、作用： 4、结果： 形成两种末端 一、 “分子手术刀” ——限制性核酸内切酶 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 内切酶切割DNA后形成的末端——黏性末端、平末端是如何形成的？ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 什么叫黏性末端？ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 什么叫平末端？ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Pro