分子生物学953分析.pptVIP

structural domains The loops of DNA between the nuclear matrix attachment points are called structural domains 染色质结构结构域：核基质附着点之间的环 Figure 8.3. A functional domain in a DNase I sensitive region. ? 2002 Garland Science functional domains : the region of DNA around an expressed gene or set of genes A functional domain is delineated by treating a region of purified chromatin with deoxyribonuclease I (DNase I) which, being a DNA-binding protein, cannot gain access to the more compacted regions of DNA. 表达的基因及其附近的区域 染色质功能结构域由DNaseI敏感性界定 Regions sensitive to DNase I extend to either side of a gene or set of genes that is being expressed, indicating that in this area the chromatin has a more open organization, although it is not clear whether this organization is the 30nm fiber or the ‘beads-on-a-string’ structure DNaseI敏感性延伸到表达基因的两侧，表明这一区域染色质结构更伸展 Figure 8.4. Insulator sequences in the fruit-fly genome. The diagram shows the region of the Drosophila genome containing the two hsp70 genes. The insulator sequences scs and scs′ are either side of the gene pair. The arrows below the two genes indicate that they lie on different strands of the double helix and so are transcribed in opposite directions. ? 2002 Garland Science Functional domains are defined by insulators The boundaries of functional domains are marked by sequences, 1–2kb in length, called insulators ( Bell et al., 2001). 功能结构域的边界由绝缘子确定 Figure 8.5. The positional effect. (A) A cloned gene that is inserted into a region of highly packaged chromatin will be inactive, but one inserted into open chromatin will be expressed. (B) The results of cloning experiments without (red) and with (blue) insulator sequences. When insulators are absent, the expression level of the cloned gene is variable, depending on whether it is inserted into packaged or open chromatin. When flanked by insulators, the expression level is consistently high because the insulators establish a functional domain at the insertion site. ?