C13-methacetin breath test.pdfVIP

13C-methacetin breath test reproducibility study reveals persistent CYP1A2 stimulation on repeat examinations Anna Kasicka-Jonderko, Anna Nita, Krzysztof Jonderko, Magdalena Kamińska, Barbara B?ońska-Fajfrowska Anna Kasicka-Jonderko, Anna Nita, Krzysztof Jonderko, Magdalena Kamińska, Barbara B?ońska-Fajfrowska, Depart- ment of Basic Biomedical Science, School of Pharmacy, Medical University of Silesia, PL-41-205 Sosnowiec, Poland Author contributions: Kasicka-Jonderko A and Jonderko K de- signed the research, analysed the data and wrote the paper; Nita A and Kamińska M performed the research, collected and analysed the data; B?ońska-Fajfrowska B was involved in providing finan- cial support for this work and approved the final version. Supported by The Medical University of Silesia, contracts No. NN-1-106-06, KNW-1-043/08 and KNW-1-154/09 Correspondence to: Dr. Anna Kasicka-Jonderko, Department of Basic Biomedical Science, School of Pharmacy, Medical Uni- versity of Silesia, Kasztanowa street 3, PL-41-205 Sosnowiec, Poland. akj@.pl Telephone: +48-32-2699830 Fax: +48-32-2699833 Received: January 12, 2011 Revised: February 15, 2011 Accepted: February 22, 2011 Published online: December 7, 2011 Abstract AIM: To find the most reproducible quantitative param- eter of a standard 13C-methacetin breath test (13C-MBT). METHODS: Twenty healthy volunteers (10 female, 10 male) underwent the 13C-MBT after intake of 75 mg 13C-methacetin p.o. on three occasions. Short- and medium-term reproducibility was assessed with paired examinations taken at an interval of 2 and 18 d (medi- ans), respectively. RESULTS: The reproducibility of the 1-h cumulative 13C recovery (AUC0-60), characterized by a coefficient of variation of 10%, appeared to be considerably better than the reproducibility of the maximum momentary 13C recovery or the time of reaching it. Remarkably, as op- posed to the short gap between consecutive examina- tions, the capacity of the liver to handle 1