alginate as wound dressings.pdf

Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with hydrosheets composed of chitin/chitosan, fucoidan, and alginate as wound dressings Kaoru Murakami, DDS1; Masayuki Ishihara, PhD, DEng2; Hiroshi Aoki, PhD3; Shingo Nakamura, PhD4; Shin-Ichiro Nakamura, MD5; Satoshi Yanagibayashi, MD5; Megumi Takikawa, MD5; Satoko Kishimoto, PhD, DVM2; Hidetaka Yokoe, PhD, DDS1; Tomoharu Kiyosawa, PhD, MD5; Yasunori Sato, PhD, DDS1 1. Department of Oral and Maxillofacial Surgery, 2. Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan, 3. Research and Development Department, Hokkaido Industrial Technology Center, Hakodate, Hokkaido, Japan, 4. Department of Surgery, and 5. Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan Reprint requests: Kaoru Murakami, DDS, Department of Oral and Maxillofacial Surgery, National Defense Medical College, 3-2 Namiki Tokorozawa, Saitama 359-8513, Japan. Tel: 181 429 95 1211; Fax: 181 429 91 1611; Email: dr22056@ndmc.ac.jp Manuscript received: October 7, 2009 Accepted in final form: May 14, 2010 DOI:10.1111/j.1524-475X.2010.00606.x ABSTRACT To create a moist environment for rapid wound healing, a hydrosheet composed of alginate, chitin/chitosan, and fucoidan (ACF-HS) has been developed as a functional wound dressing. The aim of this study was to evaluate the accelerating effect of ACF-HS on wound healing for rat mitomycin C-treated healing- impaired wounds. Full-thickness skin defects were made on the back of rats and mitomycin C was applied onto the wound for 10 minutes to prepare a healing- impaired wound. After thoroughly washing out the mitomycin C, ACF-HS was applied to the healing-impaired wounds. The rats were later euthanized and histological sections of the wounds were prepared. The histological examinations showed significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ACF-HS o