2型猪链球菌89K致病岛进化途径分析-第三军医大学学报.doc

2型猪链球菌89K致病岛进化途径分析? 胡力文,邹凌云, 倪青山，姚新月,朱军民，李明，胡福泉 （400038 重庆，第三军医大学基础部微生物学教研室） [摘要] 目的 分析89K致病岛的结构特征，探索其进化历程。 方法 通过BLAST比对，获取89K致病岛的同源序列，构建其同源序列数据库；分析89K致病岛的碱基组成特征，寻找重组热点区域，并对89K进行分区；对各ORF进行功能预测，划分功能模块；对89K致病岛与其同源序列进行系统发育分析及共线性分析，推测其可能的进化历程。 结果 89K致病岛可分为4个保守区，4个主要的非保守区及一段Tn916转座子，呈现多个异源序列相嵌合的结构特征。保守区的基因呈现出进化上的一致性，且在某些菌株中保守区以连续状态存在。结论 保守区在祖先状态时是一个连续整体，不同来源的非保守区经过多次水平转移和重组事件插入到保守区之间，形成了89K致病岛的嵌合结构。 [关键词] 致病岛；生物信息学；系统发育分析；进化途径 [中图分类号] Q939.1 Q75 [文献标志码] A Analysis of the 89K pathogenicity island evolutionary history of S.suis 2 Hu Liwen, Zou Lingyun, Ni Qingshan，Yao Xinyue，Zhu Junmin，Li Ming，Hu Fuquan (Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038) [Abstract] Objective To analysis the structure of 89K pathogenicity island (89K PAI)，and to explore its evolutionary history. Methods Running Blast aginst public database to acquire 89K PAI homogenous sequences and to construct the homogenous protein database. Analysis the base composition pattern to find recombination hotspots，and divide the 89K PAI into several independent regions. Make function prediction of the ORFs to distinguish functional modules. Make phylogenetic and co-linearity analysis with its relative sequences to speculate its possible evolutionary pathway. Results The 89K PAI is divided into four conserved regions，four major non-conserved regions and a Tn916 transposon，showing mosaic structure made up of sequences from diverse origins. Genes in conserved regions show congrudent evolutionary pattern and in some bacteria genome the conserved regions are continous. Conclusions The conserved regions existed as an entirety when ancestor status，non-conserved regions from diverse origins inserted into the conserved regions by multiple recombinant or horizontal transfer events，resulting in the mosaic structure of 89K PAI. [Key words] pathogenicity island；bioinformatics； phylogenetic analysis； evolutionary p