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厌氧消化Influence of Inoculum Source and Pre-incubation on Bio-Methane Potential
Influence of Inoculum Source and Pre-incubation
on Bio-Methane Potential of Chicken Manure
and Corn Stover
Yeqing Li Lu Feng Ruihong Zhang Yanfeng He
Xiaoying Liu Xiao Xiao Xinxin Ma Chang Chen
Guangqing Liu
Received: 17 January 2013 /Accepted: 17 June 2013 /
Published online: 2 July 2013
# Springer Science+Business Media New York 2013
Abstract In order to investigate the effects of inoculum source and pre-incubation on
methane production performance of chicken manure (CM) and corn stover (CS), two sets
of bio-methane potential tests using non- and pre-incubated inocula (digested sludge from a
municipal wastewater treatment plant (DSMW) and digested sludge from a chicken manure
treatment plant (DSCM)) were conducted at 37 °C. Modified Gompertz and first-order
models were used to evaluate the kinetic parameters. The results revealed that DSMW
was better than DSCM in digesting organic substrates (CM and CS), since the average
ultimate methane yields were 351 mL g?1 volatile solid (VS)added for CM and 300 mL g
?1
VSadded for CS when DSMW was used as inoculum, and 298 mL g
?1 VSadded for CM and
218 mL g?1 VSadded for CS when DSCM was used as inoculum, respectively. Nevertheless,
there was no significant difference (p0.05) in the ultimate methane yields between non- and
pre-incubated inoculum for digesting CM and CS, regardless of the inoculum source.
However, when evaluating the kinetic parameters of anaerobic digestion, the correlation
coefficient, maximal methane production rate, and hydrolysis rate constant were slightly
higher using pre-incubated inoculum as compared to non-incubated inoculum.
Keywords Bio-methane potential . Inoculum source . Pre-incubation . Chicken manure .
Corn stover
Appl Biochem Biotechnol (2013) 171:117–127
DOI 10.1007/s12010-013-0335-7
Yeqing Li and Lu Feng contributed to this work equally.
Y. Li : L. Feng : R. Zhang : Y. He : X. Liu :X. Xiao : X. Ma : C. Chen (*) : G. Liu (*)
Biomass Energy and Environmental Engineering Research Cen
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